WizScript™ cDNA Synthesis Kit is a complete system for the efficient synthesis of first-strand cDNA from RNA templates. The recombinant Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation. The kit is also supplied with both oligo dT and random primers. The oligo dT anneals selectively on the poly(A) tail of mRNA. Random primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5’-end regions of mRNA. Gene-specific primers may also be used with the kit. The first strand of cDNA can be directly used as a template in PCR.

• Overview

  • Total Kit for cDNA synthesis
  • Broad dynamic range
  • Highly efficient synthesis of first-strand cDNA
  • Thermostable RTase with RNase H minus
  • Wide temperature range : 42 ~ 55°C

• Ordering Information

  Cat No.	Size	Remark
  W2201	50 rxn
  W2202	100 rxn

• Features

  Contents	50 rxn	100 rxn
  WizScript™ RTase (200U/µl)	50 µl	100 µl
  10X Reaction Buffer	100 µl	200 µl
  RNase Inhibitor (40U/µl)	25 µl	50 µl
  dNTP mix (2.5mM)	100 µl	200 µl
  Oligo dT20 primer (50pM)	50 µl	100 µl
  Random hexamer (50pM)	100 µl	200 µl
  DTT (100 mM)	50 µl	100 µl
  RNase free water	1 ml	1 ml

• Technical Data

  

• Application

  • First Strand cDNA synthesis for RT-PCR
  • Construction of cDNA libraries
  • Generation of probes for hybridization
  • Antisense RNA synthesis

• Storage & Shipping

  Store at -20ºC in a frost-free freezer
  Shipped on ice packs and/or dry ice