cDNA SynthesisPCRWizScript™ cDNA Synthesis Kit

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Açıklama

WizScript™ cDNA Synthesis Kit is a complete system for the efficient synthesis of first-strand cDNA from RNA templates. The recombinant Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation. The kit is also supplied with both oligo dT and random primers. The oligo dT anneals selectively on the poly(A) tail of mRNA. Random primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5’-end regions of mRNA. Gene-specific primers may also be used with the kit. The first strand of cDNA can be directly used as a template in PCR.

  • Overview
    • Total Kit for cDNA synthesis
    • Broad dynamic range
    • Highly efficient synthesis of first-strand cDNA
    • Thermostable RTase with RNase H minus
    • Wide temperature range : 42 ~ 55°C
  • Ordering Information
    Cat No. Size Remark
    W2201 50 rxn
    W2202 100 rxn
  • Features
    Contents 50 rxn 100 rxn
    WizScript™ RTase (200U/µl) 50 µl 100 µl
    10X Reaction Buffer 100 µl 200 µl
    RNase Inhibitor (40U/µl) 25 µl 50 µl
    dNTP mix (2.5mM) 100 µl 200 µl
    Oligo dT20 primer (50pM) 50 µl 100 µl
    Random hexamer (50pM) 100 µl 200 µl
    DTT (100 mM) 50 µl 100 µl
    RNase free water 1 ml 1 ml
  • Technical Data
  • Application
    • First Strand cDNA synthesis for RT-PCR
    • Construction of cDNA libraries
    • Generation of probes for hybridization
    • Antisense RNA synthesis
  • Storage & Shipping
    Store at -20ºC in a frost-free freezer
    Shipped on ice packs and/or dry ice

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