WizScript™ RT FDmix



WizScript™ RT FDmix (dT20 or Hexamer) is a complete system for the efficient synthesis of the first-strand cDNA from RNA templates and combines all the reagents necessary for successful cDNA synthesis in a  convenient individually aliquot and lyophilized in single-tube, one-step format. The FDmix formulation saves time and reduces potential contaminating errors by eliminating several pipetting steps.
WizScript™ RT FDmix included MMLV RTase (RNase H-) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade the template during long incubation times which are required for producing long cDNAs. RNase H minus RT enables the preparation of long cDNAs and libraries containing a high percentage of full-length cDNA. An enhanced buffer allows for RT reaction temperatures up to 50°C.

  • Overview
    • Ready-to-use dried type
    • Easy reaction setup (pre-aliquot in 8-strip tube)
    • Minimal optimization requirements
    • Room-temperature reaction set-up
    • Applied unique enhancing & stabilizing technology
    • Eliminating extra pipetting steps and reducing contamination risk from
    • multiple reagents and providing better reproducibility
  • Ordering Information
Cat No. Size Remark
W2204 96 rxn Includes oligo dT20 primer
W2205 96 rxn Includes random hexamer
Technical Data


There are no reviews yet.

Be the first to review “WizScript™ RT FDmix”