Description
WizPure™ Taq DNA Polymerase is a thermally stable, processive, 5’→ 3’ DNA polymerase. The 94 kDa protein possesses an inherent 5’→3’ nick-translation moiety and lacks a 3’→5’ proofreading function.
- Overview
-
- Error rate : 2.4 X 10-5
- Thermal stability: Half-life of 40 min at 95°C
- A-tail formation at 3’ ends of amplified duplex DNA
- Recombinant proteins expressed in E. coli
- Free of detectable non-specific nuclease
- Ordering Information
-
Cat No. Size Note W1301E 500 U without dNTPs W1301E-5 2,500 U without dNTPs W1301 500 U with dNTPs W1301-5 2,500 U with dNTPs 1 unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
- Features
- 500 U contains:
- 1 x 100 µl, Taq DNA Polymerase (5U/µl)
- 2 x 1 ml, 10X PCR Buffer
- [ 1 x 1 ml, dNTP mix (2.5 mM each) ]
2,500 U contains:
- 1 x 500 µl, Taq DNA Polymerase (5U/µl)
- 10 x 1 ml, 10X PCR Buffer
- [ 5 x 1 ml, dNTP mix (2.5 mM each) ]
- Technical Data
- Application
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- Amplification of DNA fragments shorter than 3 kb (Suitable for general PCR analysis)
- Primer extension
- Colony PCR
- Multiplex PCR
- Labeling of DNA fragments with radioactive-isotopes Nucleotide sequencing
- Storage & Shipping
- Store at -20ºC in a frost-free freezer
Shipped on ice packs and/or dry ice
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