DNA PolymeraseEnzymeWizPure™ Taq DNA Polymerase

SKU: N/A

Açıklama

WizPure™ Taq DNA Polymerase is a thermally stable, processive, 5’→ 3’ DNA polymerase. The 94 kDa protein possesses an inherent 5’→3’ nick-translation moiety and lacks a 3’→5’ proofreading function.

  • Overview
  • Error rate : 2.4 X 10-5
  • Thermal stability: Half-life of 40 min at 95°C
  • A-tail formation at 3’ ends of amplified duplex DNA
  • Recombinant proteins expressed in E. coli
  • Free of detectable non-specific nuclease
  • Ordering Information
Cat No. Size   Note
W1301E 500 U without dNTPs
W1301E-5 2,500 U without dNTPs
W1301 500 U with dNTPs
W1301-5 2,500 U with dNTPs

1 unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

  • Features
500 U contains: 

  • 1 x 100 µl, Taq DNA Polymerase (5U/µl)
  • 2 x 1 ml, 10X PCR Buffer
  • [ 1 x 1 ml, dNTP mix (2.5 mM each) ]

2,500 U contains:

  • 1 x 500 µl, Taq DNA Polymerase (5U/µl)
  • 10 x 1 ml, 10X PCR Buffer
  • [ 5 x 1 ml, dNTP mix (2.5 mM each) ]
  • Technical Data
  • Application
  • Amplification of DNA fragments shorter than 3 kb (Suitable for general PCR analysis)
  • Primer extension
  • Colony PCR
  • Multiplex PCR
  • Labeling of DNA fragments with radioactive-isotopes Nucleotide sequencing
  • Storage & Shipping
Store at -20ºC in a frost-free freezer
Shipped on ice packs and/or dry ice

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