DNA PolymeraseEnzymeWizPure™ HS-Taq DNA Polymerase

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Açıklama

WizPure™ HS-Taq DNA Polymerase is an antibody-mediated hot-start Taq DNA polymerase and quite suitable for high-specific hot-start PCR, real-time PCR, and multiplex PCR. The enzyme is a highly processive 5’→3’ DNA polymerase that lacks 3’→5’ exonuclease activity and lacks a 3’→5’ proofreading function.

  • Overview
    • Error rate : 2.4 X 10-5
    • Thermal stability : Half-life of 40 min at 95°C
    • A-tail formation at 3’ ends of amplified duplex DNA
    • Recombinant proteins expressed in E. coli
    • Free of detectable non-specific nuclease
  • Ordering Information
      Cat No.      Size    Note
    W1311E 500 U without dNTPs
    W1311E-5 2,500 U without dNTPs
    W1311 500 U with dNTPs
    W1311-5 2,500 U with dNTPs

    1 unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

  • Features
    500 U: 

    • 1 x 100 µl, HS-Taq DNA Polymerase (5U/µl)
    • 2 x 1 ml, 10X PCR Buffer
    • [ 1 x 1 ml, dNTP mix (2.5 mM each) ]

    2,500 U:

    • 1 x 500 µl, HS-Taq DNA Polymerase (5U/µl)
    • 10 x 1 ml, 10X PCR Buffer
    • [ 5 x 1 ml, dNTP mix (2.5 mM each) ]
  • Technical Data
  • Application
    • Routine PCR
    • Routine diagnostic PCR requiring high reproducibility
    • DNA sequencing template preparation
  • Storage & Shipping
    Store at -20ºC in a frost-free freezer
    Shipped on ice packs and/or dry ice

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