WizPure™ HS-Taq DNA Polymerase is an antibody-mediated hot-start Taq DNA polymerase and quite suitable for high-specific hot-start PCR, real-time PCR, and multiplex PCR. The enzyme is a highly processive 5’→3’ DNA polymerase that lacks 3’→5’ exonuclease activity and lacks a 3’→5’ proofreading function.

• Overview

  • Error rate : 2.4 X 10-5
  • Thermal stability : Half-life of 40 min at 95°C
  • A-tail formation at 3’ ends of amplified duplex DNA
  • Recombinant proteins expressed in E. coli
  • Free of detectable non-specific nuclease

• Ordering Information

  Cat No.	Size	Note
  W1311E	500 U	without dNTPs
  W1311E-5	2,500 U	without dNTPs
  W1311	500 U	with dNTPs
  W1311-5	2,500 U	with dNTPs

  1 unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

• Features

  500 U: 

  • 1 x 100 µl, HS-Taq DNA Polymerase (5U/µl)
  • 2 x 1 ml, 10X PCR Buffer
  • [ 1 x 1 ml, dNTP mix (2.5 mM each) ]

  2,500 U:

  • 1 x 500 µl, HS-Taq DNA Polymerase (5U/µl)
  • 10 x 1 ml, 10X PCR Buffer
  • [ 5 x 1 ml, dNTP mix (2.5 mM each) ]

• Technical Data

  

• Application

  • Routine PCR
  • Routine diagnostic PCR requiring high reproducibility
  • DNA sequencing template preparation

• Storage & Shipping

  Store at -20ºC in a frost-free freezer
  Shipped on ice packs and/or dry ice