WizPure™ Bst DNA Polymerase (Large Fragment, exo-) is a DNA polymerase from Bacillus stearothermophilus that is modified so that it retains its 5’→ 3’ DNA polymerase activity while lacking the intrinsic 5’→ 3’ exonuclease domain. This polymerase also has strand displacement capabilities making it an ideal candidate for isothermal amplification.

• Overview

  • 5’→3′ DNA polymerase activity and strand-displacement activity
  • DNA synthesis can be performed at a constant temperature
  • Suitable for synthesis of DNA strands having high GC content
  • Not affected by inhibition due to the secondary structure of DNA
  • High purity/high-quality control

• Ordering Information

  Cat No.	Size	Remark
  W1321	2,000 U
  W1321-5	10,000 U

• Features

  2,000 U contains: 
  1 x 250 µl, Bst DNA Polymerase (8U/µl)
  2 x 1.5 ml, 10X Bst Reaction Buffer10,000 U contains: 
  1 x 1,250 µl, Bst DNA Polymerase (8U/µl)
  7 x 2 ml, 10X Bst Reaction Buffer

• Figures

  

• Applications

  • DNA sequencing through high GC regions
  • Rapid Sequencing from nanogram amounts of DNA template
  • Loop-mediated isothermal amplification (LAMP)
  • Whole genome amplification (WGA)
  • Ramification amplification (RAM)

• Storage & Shipping

  Store at -20ºC in a frost-free freezer
  Shipped on ice packs and/or dry ice