WizPure™ RT-PCR FDmix combines all the reagents necessary for successful routine RT-PCR in a convenient individually aliquot and lyophilized in single-tube, one-step format. The premixed formulation saves time and reduces potential contaminating errors by eliminating several pipetting steps.
WizPure™ RT-PCR FDmix produces fast, highly-specific, and ultra-sensitive one-step RT-PCR, from either total RNA or mRNA using gene-specific primers. An enhanced buffer allows for RT reaction temperatures up to 50°C. This can improve the detection of more difficult targets as higher RT temperatures reduce nonspecific priming and facilitate the melting of RNA secondary structures.
In addition, the RT-PCR FDmix includes the MMLV RTase (RNase H-) and antibody-based hot-start Taq DNA polymerase, which has been shown to improve the sensitivity and specificity for certain targets.

• Overview

• Including loading dye for easy gel electrophoresis
• A-tail formation at 3’ ends of amplified duplex DNA
• Ready-to-use dried type
• Easy reaction setup (pre-aliquot in 8-strip tube)
• High PCR specificity and high sensitivity PCR amplification
• Room-temperature reaction set-up
• Contains loading dye for visual confirmation and quick electrophoresis
• Minimal optimization requirements
• Eliminating extra pipetting steps and reducing contamination risk from multiple reagents and providing better reproducibility

• Ordering Information

Cat No.	Size	Remark
W2601	96 T	8 strip x 12 ea
W2601-5	480 T	8 strip x 60 ea

• Technical Data

• Applications

• High through-put RT-PCR
• Routine RT-PCR amplification of RNA templates
• Multiple band detection or genotyping

• Storage & Shipping

Shipped at room temperature
Store at -20ºC, upon receipt