EnzymeModifying EnzymeProteinase K, Recombinant

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Açıklama

Proteinase K is a subtilisin-related serine protease that will hydrolyze a variety of peptide bonds. Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 60°C and a pH between 7.5 and 12.0. Activity is stimulated when up to 2% SDS or up to 4 M urea are included in the reaction. Calcium is important for thermostability of Proteinase K but it is not required for catalysis, therefore Proteinase K is also active in buffers containing chelating agents such as EDTA.

  • Overview
    • Form : White to Light Yellow Powder, Lyophilized from purified liquid enzyme
    • Source : Mutated gene from Tritirachium album limber, Recombinant
    • Specific Activity : ≥30U/mg dry weights
    • Molecular Weight : 29.3 kDa
  • Ordering Information
    Cat No. Size Remark
    W6050 22 mg
  • Applications
    • Isolation of plasmid and genomic DNA
    • Isolation of RNA
    • Inactivation of RNases, DNases and enzymes in reactions
    • Removal of enzymes from DNA to improve cloning efficiency
    • PCR purification
  • Storage & Shipping
    Store at 4 to -20ºC recommended; Mutated Proteinase K powder is stable at room temperature in two years.

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