Proteinase K is a subtilisin-related serine protease that will hydrolyze a variety of peptide bonds. Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 60°C and a pH between 7.5 and 12.0. Activity is stimulated when up to 2% SDS or up to 4 M urea are included in the reaction. Calcium is important for thermostability of Proteinase K but it is not required for catalysis, therefore Proteinase K is also active in buffers containing chelating agents such as EDTA.

• Overview

  • Form : White to Light Yellow Powder, Lyophilized from purified liquid enzyme
  • Source : Mutated gene from Tritirachium album limber, Recombinant
  • Specific Activity : ≥30U/mg dry weights
  • Molecular Weight : 29.3 kDa

• Ordering Information

  Cat No.	Size	Remark
  W6050	22 mg

• Applications

  • Isolation of plasmid and genomic DNA
  • Isolation of RNA
  • Inactivation of RNases, DNases and enzymes in reactions
  • Removal of enzymes from DNA to improve cloning efficiency
  • PCR purification

• Storage & Shipping

  Store at 4 to -20ºC recommended; Mutated Proteinase K powder is stable at room temperature in two years.