EnzymeReverse TranscriptaseWizScript™ III Reverse Transcriptase

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Açıklama

WizScript™ Reverse Transcriptase is a genetically modified M-MLV Reverse Transcriptase that exhibits RNA or DNA-dependent DNA polymerase but lacks ribonuclease H activity.
This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products. The RNase H activity degrades the RNA part of the cDNA/RNA hybrid following reverse transcription, which may prevent its inhibition during subsequent PCR steps.

  • Overview
    • RNase H negative
    • Molecular weight : 71 kDa
    • Reaction temperature : 37°C ~ 42°C
    • Heat inactivation : 70°C / 10 min.
    • Free of endo- and exodeoxyribonucleases, phosphatases, and ribonuclease
    • 200 units/μl
  • Ordering Information
    Cat No. Size Remark
    W2303 10,000 U 200U/μl
    W2303-5 50,000 U 200U/μl
  • Features
    10,000 U contains: 
    1 x 50 μl, WizScript™ III Reverse Transcriptase
    1 x 500 μl, 5X Reaction Buffer [250 mM Tris-HCl, 375 mM KCl, 15 mM MgCl2, 50 mM dithiothreitol, pH 8.3 (25°C
    )]

    50,000 U contains:
    5 x 50 μl, WizScript™ III Reverse Transcriptase
    5 x 500 μl, 5X Reaction Buffer

  • Technical Data
  • Application
    • Gene synthesis
    • First-strand synthesis of cDNA
    • RT-PCR
    • Random priming reactions
    • Library construction
    • Probe labeling
    • mRNA 5’-end mapping
  • Storage & Shipping
    Store at -20ºC in a frost-free freezer
    Shipped on ice packs and/or dry ice

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