WizScript™ Reverse Transcriptase is a genetically modified M-MLV Reverse Transcriptase that exhibits RNA or DNA-dependent DNA polymerase but lacks ribonuclease H activity.
This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products. The RNase H activity degrades the RNA part of the cDNA/RNA hybrid following reverse transcription, which may prevent its inhibition during subsequent PCR steps.

• Overview

  • RNase H negative
  • Molecular weight : 71 kDa
  • Reaction temperature : 37°C ~ 42°C
  • Heat inactivation : 70°C / 10 min.
  • Free of endo- and exodeoxyribonucleases, phosphatases, and ribonuclease
  • 200 units/μl

• Ordering Information

  Cat No.	Size	Remark
  W2303	10,000 U	200U/μl
  W2303-5	50,000 U	200U/μl

• Features

  10,000 U contains: 
  1 x 50 μl, WizScript™ III Reverse Transcriptase
  1 x 500 μl, 5X Reaction Buffer [250 mM Tris-HCl, 375 mM KCl, 15 mM MgCl2, 50 mM dithiothreitol, pH 8.3 (25°C
  )]

  50,000 U contains:
  5 x 50 μl, WizScript™ III Reverse Transcriptase
  5 x 500 μl, 5X Reaction Buffer

• Technical Data

  

• Application

  • Gene synthesis
  • First-strand synthesis of cDNA
  • RT-PCR
  • Random priming reactions
  • Library construction
  • Probe labeling
  • mRNA 5’-end mapping

• Storage & Shipping

  Store at -20ºC in a frost-free freezer
  Shipped on ice packs and/or dry ice