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WizPure™ HS-PCR FDmix combines all the reagents necessary for successful antibody-based hot-start PCR in a convenient individually aliquoted and lyophilized in single-tube. WizPure™ HS-PCR FDmix is an optimized, economical, highly efficient, and ready-to-use hot-start PCR premix that can amplify templates of up to 5 kb. The PCR mixture of hot-start Taq DNA Polymerase, PCR buffer, MgCl2, and dNTPs. This HS-PCR FDmix contains all components for PCR, except DNA template and primers. The mixture is suitable for amplification of most of the DNA templates. Tested for the absence of endonucleases & exonucleases and is also tested for amplification of single gene copy.
WizPure™ HS-PCR FDmix yields excellent and consistent results in routine PCR reactions as well as high-throughput PCR genotyping, colony PCR, RT-PCR, and PCR cloning. PCR reactions can be directly loaded onto an agarose gel without the additional need of loading buffer and dyes.
- Overview
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- Including loading dye for easy gel electrophoresis
- A-tail formation at 3’ ends of amplified duplex DNA
- Ready-to-use dried type
- Easy reaction setup (pre-aliquot in 8-strip tube)
- High PCR specificity and high sensitivity PCR amplification
- Antibody-mediated hot-start PCR
- Room-temperature reaction set-up
- Contains loading dye for visual confirmation and quick electrophoresis
- Minimal optimization requirements
- Eliminating extra pipetting steps and multiple reagents reduces contamination risk and provide better reproducibility
- Ordering Information
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Cat No. Size Remark W1611 96 T 8 strip x 12 ea W1611-5 480 T 8 strip x 60 ea
- Technical Data
- Applications
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- High through-put PCR
- Routine diagnostic PCR requiring high reproducibility
- Hot-start PCR
- Primer extension
- Multiplex PCR
- High specific amplification of DNA fragments shorter than 3kb
- Storage & Shipping
- Shipped at room temperature
Store at -20ºC, upon receipt
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