WizPure™ FX-PCR 2X Master is a ready-to-use and complete system for rapid, consistent, and accurate amplification of long PCR products (>5~30 kb). This kit optimized for PCR amplification of genomic DNA templates up to 20 kb and lambda DNA up to 30 kb. With its enhanced processivity, yield, speed, and excellent 3’→5’ exonuclease and 3′→5′ proofreading activity, this enzyme are able to consistently deliver accurate and reliable amplification of long templates.
This product is the ideal choice for long DNA templates unable to be amplified in conventional PCR and is highly suitable for multiple downstream applications including complex cloning and genotyping experiments. The PCR product amplified with this mixture has one A added at 3’-end, so the product can be directly used for TA cloning.

• Overview

• Including loading dye for easy gel electrophoresis
• A-tail formation at 3’ ends of amplified duplex DNA
• Ready-to-use solution
• Easy reaction setup
• Long and high fidelity PCR amplification
• Room-temperature reaction set-up
• Contains loading dye for visual confirmation and quick electrophoresis
• Minimal optimization requirements

• Ordering Information

Cat No.	Size	Remark
W1461	1 ml	100 rxn
W1461-5	5 x 1 ml	500 rxn

• Technical Data

• Applications

• Long range PCR
• High-fidelity PCR
• Primer-extension reactions
• Genotyping
• Library contsruction
• High GC amplification
• Next-generation DNA sequencing
• TA cloning

• Storage & Shipping

Store at -20ºC in a frost-free freezer
Shipped on ice packs and/or dry ice