EnzymeReverse TranscriptaseWizPure™ MMLV RTase (RNase H+)

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Açıklama

WizPure™ MMLV RTase (RNase H+) encoded by Moloney Murine Leukemia Virus (MMLV) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized.
This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. MMLV RTase will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.

  • Overview
    • 5′ to 3′ exonuclease: No
    • 3′ to 5′ exonuclease: No
    • 3’ – A Overhang: No
    • Fragment Size: Up to 10 kb
    • Free of endo- and exodeoxyribonucleases, phosphatases, and ribonuclease
    • 200 units/μl
  • Ordering Information
    Cat No. Size Remark
    W2301 10,000 U 200U/μl
    W2301-5 50,000 U 200U/μl

    1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.

  • Features
    10,000 U contains: 
    1 x 50 μl, MMLV RTase (RNase H+)
    1 x 500 μl, 5X Reaction Buffer

    50,000 U contains: 
    1 x 250 μl, MMLV RTase (RNase H+)
    1 x 2,500 μl, 5X Reaction Buffer

  • Technical Data
  • Application
    • Gene synthesis
    • First-strand synthesis of cDNA
    • RT-PCR
    • Random priming reactions
    • Library construction
    • Probe labeling
    • mRNA 5’-end mapping
  • Storage & Shipping
    Store at -20ºC in a frost-free freezer
    Shipped on ice packs and/or dry ice

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