
Açıklama
WizPure™ MMLV RTase (RNase H+) encoded by Moloney Murine Leukemia Virus (MMLV) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized.
This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. MMLV RTase will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.
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Overview
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- 5′ to 3′ exonuclease: No
- 3′ to 5′ exonuclease: No
- 3’ – A Overhang: No
- Fragment Size: Up to 10 kb
- Free of endo- and exodeoxyribonucleases, phosphatases, and ribonuclease
- 200 units/μl
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Ordering Information
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Cat No. Size Remark W2301 10,000 U 200U/μl W2301-5 50,000 U 200U/μl 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.
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Features
- 10,000 U contains:
1 x 50 μl, MMLV RTase (RNase H+)
1 x 500 μl, 5X Reaction Buffer50,000 U contains:
1 x 250 μl, MMLV RTase (RNase H+)
1 x 2,500 μl, 5X Reaction Buffer
- 10,000 U contains:
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Technical Data
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Application
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- Gene synthesis
- First-strand synthesis of cDNA
- RT-PCR
- Random priming reactions
- Library construction
- Probe labeling
- mRNA 5’-end mapping
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Storage & Shipping
- Store at -20ºC in a frost-free freezer
Shipped on ice packs and/or dry ice
- Store at -20ºC in a frost-free freezer
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