WizPure™ MMLV RTase (RNase H+) encoded by Moloney Murine Leukemia Virus (MMLV) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized.
This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. MMLV RTase will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.

• Overview

  • 5′ to 3′ exonuclease: No
  • 3′ to 5′ exonuclease: No
  • 3’ – A Overhang: No
  • Fragment Size: Up to 10 kb
  • Free of endo- and exodeoxyribonucleases, phosphatases, and ribonuclease
  • 200 units/μl

• Ordering Information

  Cat No.	Size	Remark
  W2301	10,000 U	200U/μl
  W2301-5	50,000 U	200U/μl

  1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.

• Features

  10,000 U contains: 
  1 x 50 μl, MMLV RTase (RNase H+)
  1 x 500 μl, 5X Reaction Buffer

  50,000 U contains: 
  1 x 250 μl, MMLV RTase (RNase H+)
  1 x 2,500 μl, 5X Reaction Buffer

• Technical Data

  

• Application

  • Gene synthesis
  • First-strand synthesis of cDNA
  • RT-PCR
  • Random priming reactions
  • Library construction
  • Probe labeling
  • mRNA 5’-end mapping

• Storage & Shipping

  Store at -20ºC in a frost-free freezer
  Shipped on ice packs and/or dry ice