CrystalMix^® qPCR-UDG (PROBE) kit combines all the reagents necessary for successful routine real-time PCR in a convenient individually aliquot in an 8-strip qPCR tube. CrystalMix^® qPCR-UDG (PROBE) kit is an economical, highly efficient, ready-to-use, and room temperature stable format. There is no need for freezing, thawing steps, or pipetting on ice, so minimized the risk of human errors and contaminations.
CrystalMix^® qPCR-UDG (PROBE) kit contains antibody-mediated hot-start Taq DNA Polymerase, PCR buffer, MgCl2, and dATP, dCTP, dGTP, dUTP, and Uracil DNA Glycosylase (UDG). UDG and dUTP are included in the mixture to prevent the reamplification of carryover PCR products between reactions. dUTP in the mix ensures that any amplified DNA will contain uracil. UDG removes uracil residues from single- or double-stranded DNA, preventing dU-containing DNA from serving as a template in future PCRs.

• Overview

  

• Ordering Information

  Cat No.	Size	Remark
  W8702-96L	96 rxn	0.1 ml, low profile
  W8702-96H	96 rxn	0.2 ml, high profile

• Features

  Pre-aliquoted & lyophilized in 8 strip clear tube

• Technical Data

  

• Applications

  • High through-put Real-time PCR
  • Gene expression profiling
  • Gene knockdown verification
  • Array validation
  • Routine diagnostic PCR requiring high reproducibility
  • Point-of-care molecular diagnostics

• Storage & Shipping

  Store below 25°C in airtight pouch with desiccants

• Advantages

  • Room temperature stable for over 1 year
  • Ready-to-use lyophilized type
  • Fast protocol, just add sample & primer
  • No freezing/thawing step
  • No PCR mixture preparation step
  • No cold chain shipping, Simple transport logistics
  • Minimize human error and contamination
  • Maximize Stability, Reproducibility, and Reliability
  • Aliquot per each reaction
  • Eliminating extra pipetting steps and reducing contamination risk from multiple reagents and providing better reproducibility