CrystalMix^® qRT-PCR (PROBE) kit combines all the reagents necessary for successful one-step real-time RT-PCR in a convenient individually aliquot in an 8-strip qPCR tube. CrystalMix^® qRT-PCR (PROBE) kit is an economical, highly efficient, ready-to-use, and room temperature stable format. There is no need for freezing, thawing steps, or pipetting on ice, so minimized the risk of human errors and contaminations.
CrystalMix^® qRT-PCR (PROBE) kit is an optimized, ready-to-use RT-PCR mixture of antibody-mediated hot-start Taq DNA Polymerase, WizScript™ RTase, Reaction buffer, MgCl2, and dNTP’s, except DNA template and primers.  An enhanced buffer allows for RT reaction temperatures up to 50°C. This can improve the detection of more difficult targets as higher RT temperatures reduce nonspecific priming and facilitate the melting of RNA secondary structures.

• Overview

  

• Ordering Information

  Cat No.	Size	Remark
  W8721-96L	96 rxn	0.1 ml, low profile
  W8721-96H	96 rxn	0.2 ml, high profile

• Features

  Pre-aliquoted & lyophilized in 8 strip clear tube

• Technical Data

  

• Applications

  • High through-put Real-time PCR
  • Gene expression profiling
  • Gene knockdown verification
  • Array validation
  • Routine diagnostic PCR requiring high reproducibility
  • Point-of-care molecular diagnostics

• Storage & Shipping

  Store below 25°C in airtight pouch with desiccants

• Advantages

  • Room temperature stable for over 1 year
  • Ready-to-use lyophilized type
  • Fast protocol, just add sample & primer
  • No freezing/thawing step
  • No PCR mixture preparation step
  • No cold chain shipping, Simple transport logistics
  • Minimize human error and contamination
  • Maximize Stability, Reproducibility, and Reliability
  • Aliquot per each reaction
  • Eliminating extra pipetting steps and reducing contamination risk from multiple reagents and providing better reproducibility