CrystalMix® qRT-PCR (PROBE)



CrystalMix® qRT-PCR (PROBE) kit combines all the reagents necessary for successful one-step real-time RT-PCR in a convenient individually aliquot in an 8-strip qPCR tube. CrystalMix® qRT-PCR (PROBE) kit is an economical, highly efficient, ready-to-use, and room temperature stable format. There is no need for freezing, thawing steps, or pipetting on ice, so minimized the risk of human errors and contaminations.
CrystalMix® qRT-PCR (PROBE) kit is an optimized, ready-to-use RT-PCR mixture of antibody-mediated hot-start Taq DNA Polymerase, WizScript™ RTase, Reaction buffer, MgCl2, and dNTP’s, except DNA template and primers.  An enhanced buffer allows for RT reaction temperatures up to 50°C. This can improve the detection of more difficult targets as higher RT temperatures reduce nonspecific priming and facilitate the melting of RNA secondary structures.

  • Overview
  • Ordering Information
    Cat No. Size Remark
    W8721-96L 96 rxn 0.1 ml, low profile
    W8721-96H 96 rxn 0.2 ml, high profile
  • Features
    Pre-aliquoted & lyophilized in 8 strip clear tube
  • Technical Data
  • Applications
    • High through-put Real-time PCR
    • Gene expression profiling
    • Gene knockdown verification
    • Array validation
    • Routine diagnostic PCR requiring high reproducibility
    • Point-of-care molecular diagnostics
  • Storage & Shipping
    Store below 25°C in airtight pouch with desiccants
  • Advantages
    • Room temperature stable for over 1 year
    • Ready-to-use lyophilized type
    • Fast protocol, just add sample & primer
    • No freezing/thawing step
    • No PCR mixture preparation step
    • No cold chain shipping, Simple transport logistics
    • Minimize human error and contamination
    • Maximize Stability, Reproducibility, and Reliability
    • Aliquot per each reaction
    • Eliminating extra pipetting steps and reducing contamination risk from multiple reagents and providing better reproducibility


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