WizPure™ FX-Taq DNA Polymerase is a thermally stable, processive, 5’→ 3’ DNA polymerase and 3’→5’ proofreading function. The FX-Taq DNA polymerase optimized for PCR amplification of genomic DNA templates up to 20 kb and lambda DNA up to 30 kb. With its enhanced processivity, yield, speed, and excellent 3’→5’ exonuclease and 3′→5′ proofreading activity, this enzyme are able to consistently deliver accurate and reliable amplification of long templates.
This product is the ideal choice for long DNA templates unable to be amplified in conventional PCR and is highly suitable for multiple downstream applications including complex cloning and genotyping experiments. The PCR product amplified with this mixture has one A added at 3’-end, so the product can be directly used for TA cloning.

• Overview

  • Error rate : 1.3 x 10-6
  • Thermal stability : Half-life of 4 min at 95°C
  • A-tail formation at 3′ ends of amplified duplex DNA
  • Recombinant proteins expressed in E. coli
  • Free of detectable non-specific nuclease

• Ordering Information

  Cat No.	Size	Remark
  W1341ES	250 U	without dNTPs
  W1341E	500 U	without dNTPs
  W1341E-5	2,500 U	without dNTPs
  W1341S	250 U	with dNTPs
  W1341	500 U	with dNTPs
  W1341-5	2,500 U	with dNTPs

  1 unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

• Features

  250 U contains: 

  • 1 x 100 µl, FX-Taq DNA Polymerase (2.5U/µl)
  • 1 x 1 ml, 10X PCR Buffer
  • 1 x 0.5 ml, 25 mM MgCl2
  • [ 1 x 0.5 ml, dNTP mix (2.5 mM each) ]

  500 U contains: 

  • 1 x 200 µl, FX-Taq DNA Polymerase (2.5U/µl)
  • 2 x 1 ml, 10X PCR Buffer
  • 1 x 1 ml, 25 mM MgCl2
  • [ 1 x 1 ml, dNTP mix (2.5 mM each) ]

  2,500 U contains: 

  • 1 x 1,000 µl, FX-Taq DNA Polymerase (2.5U/µl)
  • 10 x 1 ml, 10X PCR Buffer
  • 5 x 1 ml, 25 mM MgCl2
  • [ 5 x 1 ml, dNTP mix (2.5 mM each) ]

• Technical Data

  

• Applications

  • Long range PCR
  • High-fidelity PCR
  • Primer-extension reaction
  • Genotyping
  • Library construction
  • High GC amplification
  • Next-generation DNA sequencing
  • TA cloning

• Storage & Shipping

  Store at -20ºC in a frost-free freezer
  Shipped on ice packs and/or dry ice