DNA PolymeraseEnzymeWizPure™ Pfu DNA Polymerase

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WizPure™ Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3′ direction.
Pfu DNA Polymerase also possesses 3’→5’exonuclease (proofreading) activity. WizPure™ Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, is even up to tenfold more accurate than normal Taq DNA polymerase. Consequently, WizPure™ Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.

  • Overview
    • Error rate : 1.3 x 10-6
    • Time to 1kb (72°C) : 60-120
    • Thermal stability : Half-life of 4 hrs at 95°C
    • Blunt end products
    • Recombinant proteins expressed in E. coli
    • Free of detectable non-specific nuclease
  • Ordering Information
    Cat No. Size Remark
    W1330E 250 U without dNTPs
    W1331E 500 U without dNTPs
    W1331E-5 2,500 U without dNTPs
    W1330 250 U with dNTPs
    W1331 500 U with dNTPs
    W1331-5 2,500 U with dNTPs

    Unit Definition 1 unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.

  • Features
    250 U contains: 

    • 1 x 100 µl, Pfu Polymerase (2.5U/µl)
    • 1 x 1 ml, 10X PCR Buffer
    • [ 1 x 0.5 ml, dNTP mix (2.5 mM each) ]

    500 U contains: 

    • 1 x 200 µl, Pfu Polymerase (2.5U/µl)
    • 2 x 1 ml, 10X PCR Buffer
    • [ 1 x 1 ml, dNTP mix (2.5 mM each) ]

    2,500 U contains: 

    • 1 x 1,000 µl, Pfu Polymerase (2.5U/µl)
    • 8 x 1 ml, 10X PCR Buffer
    • [ 5 x 1 ml, dNTP mix (2.5 mM each) ]
  • Technical Data
  • Applications
    • High fidelity PCR for cloning
    • Amplification of long DNA fragments (>5-15 kb)
    • DNA amplification to generate blunt end products
    • Site-directed mutagenesis
    • DNA sequencing
  • Storage & Shipping
    Store at -20ºC in a frost-free freezer
    Shipped on ice packs and/or dry ice

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