WizPure™ Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3′ direction.
Pfu DNA Polymerase also possesses 3’→5’exonuclease (proofreading) activity. WizPure™ Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, is even up to tenfold more accurate than normal Taq DNA polymerase. Consequently, WizPure™ Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.

• Overview

  • Error rate : 1.3 x 10-6
  • Time to 1kb (72°C) : 60-120
  • Thermal stability : Half-life of 4 hrs at 95°C
  • Blunt end products
  • Recombinant proteins expressed in E. coli
  • Free of detectable non-specific nuclease

• Ordering Information

  Cat No.	Size	Remark
  W1330E	250 U	without dNTPs
  W1331E	500 U	without dNTPs
  W1331E-5	2,500 U	without dNTPs
  W1330	250 U	with dNTPs
  W1331	500 U	with dNTPs
  W1331-5	2,500 U	with dNTPs

  Unit Definition 1 unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.

• Features

  250 U contains: 

  • 1 x 100 µl, Pfu Polymerase (2.5U/µl)
  • 1 x 1 ml, 10X PCR Buffer
  • [ 1 x 0.5 ml, dNTP mix (2.5 mM each) ]

  500 U contains: 

  • 1 x 200 µl, Pfu Polymerase (2.5U/µl)
  • 2 x 1 ml, 10X PCR Buffer
  • [ 1 x 1 ml, dNTP mix (2.5 mM each) ]

  2,500 U contains: 

  • 1 x 1,000 µl, Pfu Polymerase (2.5U/µl)
  • 8 x 1 ml, 10X PCR Buffer
  • [ 5 x 1 ml, dNTP mix (2.5 mM each) ]

• Technical Data

  

• Applications

  • High fidelity PCR for cloning
  • Amplification of long DNA fragments (>5-15 kb)
  • DNA amplification to generate blunt end products
  • Site-directed mutagenesis
  • DNA sequencing

• Storage & Shipping

  Store at -20ºC in a frost-free freezer
  Shipped on ice packs and/or dry ice