WizPure™ qPCR Master (SYBR) with ROX Dye is an optimized ready-to-use solution for real-time quantitative PCR assays. It comprises all the components necessary to perform qPCR: antibody-mediated hot-start Taq DNA Polymerase, ultra-pure dNTPs, MgCl2, SYBR Green I, enhancer, and stabilizer. Hot start DNA Polymerase is activated by a 5-minute incubation step at 95°C. This prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. The kit includes the components necessary for performing PCR amplification and has been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA, and plasmid DNA.
WizPure™ qPCR Master (SYBR) Lo-ROX kit and WizPure™ qPCR Master (SYBR) Hi-ROX kit are o

ptimized ready-to-use 2X master mix solution for real-time quantitative PCR assays. It comprises all the components necessary to perform qPCR: antibody hot-start Taq DNA Polymerase, ultrapure dNTPs, MgCl2, SYBR Green I, enhancer, stabilizer, and ROX reference dye ensures that the kits deliver fast, highly-specific and ultra-sensitive real-time PCR.

• Overview

  • Ready-to-use solution
  • Easy reaction setup
  • Minimal optimization requirements
  • Antibody-mediated hot-start PCR
  • For universal Real-time PCR master mix
  • Suitable for most Real-time PCR machine
  • Applied unique enhancing & stabilizing technology

• Ordering Information

  Cat No.	Size	Remark
  W1711	1 ml	100 rxn
  W1711-5	5 x 1 ml	500 rxn
  W1711R	1 ml	100 rxn with ROX dye
  W1711R-4	4 x 1 ml	400 rxn with ROX dye
  W1711RL	1 ml	Low ROX dye
  W1711RL-5	5 x 1 ml	Low ROX dye
  W1711RH	1 ml	High ROX dye
  W1711RH-5	5 x 1 ml	High ROX dye

• Features

  W1711:
  1 ml, qPCR Master (SYBR)

  W1711-5:
  5 x 1 ml, qPCR Master (SYBR)

  W1711R:
  1 ml, qPCR Master (SYBR)
  50 μl, ROX Dye (50X)

  W1711R-4:
  4 x 1 ml, qPCR Master (SYBR)
  200 μl, ROX Dye (50X)

  W1711RL:
  1 ml, qPCR Master (SYBR) Lo-ROX

  W1711RL-5:
  5 x 1 ml, qPCR Master (SYBR) Lo-ROX

  W1711RH:
  1 ml, qPCR Master (SYBR) Hi-ROX

  W1711RH-5:
  5 x 1 ml, qPCR Master (SYBR) Hi-ROX

• Technical Data

  

• Applications

  • High through-put Real-time PCR
  • Detection and quantification of DNA and cDNA targets
  • Gene expression profiling
  • Gene knockdown verification
  • Microbial detection
  • Viral load determination
  • Array validation
  • SNP genotyping

• Storage & Shipping

  Store at -20ºC in a frost-free freezer
  Shipped on ice packs and/or dry ice