Real-Time PCRReal-Time PCRWizPure™ qPCR Master (SYBR)

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Açıklama

WizPure™ qPCR Master (SYBR) with ROX Dye is an optimized ready-to-use solution for real-time quantitative PCR assays. It comprises all the components necessary to perform qPCR: antibody-mediated hot-start Taq DNA Polymerase, ultra-pure dNTPs, MgCl2, SYBR Green I, enhancer, and stabilizer. Hot start DNA Polymerase is activated by a 5-minute incubation step at 95°C. This prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. The kit includes the components necessary for performing PCR amplification and has been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA, and plasmid DNA.
WizPure™ qPCR Master (SYBR) Lo-ROX kit and WizPure™ qPCR Master (SYBR) Hi-ROX kit are o

ptimized ready-to-use 2X master mix solution for real-time quantitative PCR assays. It comprises all the components necessary to perform qPCR: antibody hot-start Taq DNA Polymerase, ultrapure dNTPs, MgCl2, SYBR Green I, enhancer, stabilizer, and ROX reference dye ensures that the kits deliver fast, highly-specific and ultra-sensitive real-time PCR.

  • Overview
    • Ready-to-use solution
    • Easy reaction setup
    • Minimal optimization requirements
    • Antibody-mediated hot-start PCR
    • For universal Real-time PCR master mix
    • Suitable for most Real-time PCR machine
    • Applied unique enhancing & stabilizing technology
  • Ordering Information
    Cat No. Size Remark
    W1711 1 ml 100 rxn
    W1711-5 5 x 1 ml 500 rxn
    W1711R 1 ml 100 rxn with ROX dye
    W1711R-4 4 x 1 ml 400 rxn with ROX dye
    W1711RL 1 ml Low ROX dye
    W1711RL-5 5 x 1 ml Low ROX dye
    W1711RH 1 ml High ROX dye
    W1711RH-5 5 x 1 ml High ROX dye
  • Features
    W1711:
    1 ml, qPCR Master (SYBR)

    W1711-5:
    5 x 1 ml, qPCR Master (SYBR)

    W1711R:
    1 ml, qPCR Master (SYBR)
    50 μl, ROX Dye (50X)

    W1711R-4:
    4 x 1 ml, qPCR Master (SYBR)
    200 μl, ROX Dye (50X)

    W1711RL:
    1 ml, qPCR Master (SYBR) Lo-ROX

    W1711RL-5:
    5 x 1 ml, qPCR Master (SYBR) Lo-ROX

    W1711RH:
    1 ml, qPCR Master (SYBR) Hi-ROX

    W1711RH-5:
    5 x 1 ml, qPCR Master (SYBR) Hi-ROX

  • Technical Data
  • Applications
    • High through-put Real-time PCR
    • Detection and quantification of DNA and cDNA targets
    • Gene expression profiling
    • Gene knockdown verification
    • Microbial detection
    • Viral load determination
    • Array validation
    • SNP genotyping
  • Storage & Shipping
    Store at -20ºC in a frost-free freezer
    Shipped on ice packs and/or dry ice

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