WizPure™ qPCR Master (EVA)



WizPure™ qPCR Master (EVA) is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen dye. It comprises all the components necessary to perform qPCR: WizPure™ HS-Taq DNA Polymerase, ultrapure dNTPs, MgCl2, and EvaGreen dye. The user simply needs to add water, template, and primers. Hot start DNA Polymerase is activated by a 5 minutes incubation step at 95°C. This prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
The kit includes the components necessary for performing PCR amplification and has been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA, and plasmid DNA.

  • Overview
    • Ready-to-use solution
    • Easy reaction setup
    • Minimal optimization requirements
    • Antibody-mediated hot-start PCR
    • For universal Real-time PCR master mix
    • Suitable for most Real-time PCR machine
    • Applied unique enhancing & stabilizing technology
  • Ordering Information
    Cat No. Size Remark
    W1721 1 ml 100 rxn
    W1721-5 5 x 1 ml 500 rxn
    W1721R 1 ml 100 rxn with ROX dye
    W1721R-4 4 x 1 ml 400 rxn with ROX dye
  • Features
    1 ml, qPCR Master (EVA)

    5 x 1 ml, qPCR Master (EVA)

    1 ml, qPCR Master (EVA)
    50 μl, ROX Dye (50X)

    4 x 1 ml, qPCR Master (EVA)
    200 μl, ROX Dye (50X)

  • Technical Data
  • Applications
    • Real-time PCR
    • Detection and quantification of DNA and cDNA targets
    • Gene expression profiling & knockdown verification
    • Microbial detection
    • Viral load determination
    • Array validation
    • SNP genotyping
  • Storage & Shipping
    Store at -20ºC in a frost-free freezer
    Shipped on ice packs and/or dry ice


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