WizPure™ qPCR Master (EVA) is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen dye. It comprises all the components necessary to perform qPCR: WizPure™ HS-Taq DNA Polymerase, ultrapure dNTPs, MgCl2, and EvaGreen dye. The user simply needs to add water, template, and primers. Hot start DNA Polymerase is activated by a 5 minutes incubation step at 95°C. This prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
The kit includes the components necessary for performing PCR amplification and has been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA, and plasmid DNA.

• Overview

  • Ready-to-use solution
  • Easy reaction setup
  • Minimal optimization requirements
  • Antibody-mediated hot-start PCR
  • For universal Real-time PCR master mix
  • Suitable for most Real-time PCR machine
  • Applied unique enhancing & stabilizing technology

• Ordering Information

  Cat No.	Size	Remark
  W1721	1 ml	100 rxn
  W1721-5	5 x 1 ml	500 rxn
  W1721R	1 ml	100 rxn with ROX dye
  W1721R-4	4 x 1 ml	400 rxn with ROX dye

• Features

  W1721:
  1 ml, qPCR Master (EVA)

  W1721-5:
  5 x 1 ml, qPCR Master (EVA)

  W1721R:
  1 ml, qPCR Master (EVA)
  50 μl, ROX Dye (50X)

  W1721R-4:
  4 x 1 ml, qPCR Master (EVA)
  200 μl, ROX Dye (50X)

• Technical Data

  

• Applications

  • Real-time PCR
  • Detection and quantification of DNA and cDNA targets
  • Gene expression profiling & knockdown verification
  • Microbial detection
  • Viral load determination
  • Array validation
  • SNP genotyping

• Storage & Shipping

  Store at -20ºC in a frost-free freezer
  Shipped on ice packs and/or dry ice