WizPrep™ Gel/PCR Purification Mini Kit was designed to recover or concentrate DNA fragments (100 bp→10 Kb) from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to dissolve agarose gel and denature enzymes.
DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. The unique dual-purpose application and high yield DNA/ minicolumn make this kit an exceptional value.
The method uses a chaotropic salt, guanidine thiocyanate to dissolve agarose gel and denature enzymes. DNA fragments in chaotropic salt solution bind to the glass fiber matrix of the spin column. Following washing off of contaminants, the purified DNA fragments are eluted by the addition of low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without phenol extraction or alcohol precipitation.

• Overview

  • Optimized for fast and efficient DNA isolation based on silica membrane spin columns
  • High Efficiency : up to 90% recovery from agarose gels up to 95% recovery from PCR products or other enzymatic reactions
  • Convenient : gel extraction and PCR cleanup in one
  • Fast protocol : 10 minute
  • Broad Fragment Size Range: 100 bp ~ 20 kb
  • pH indicator is included in the GP Buffer to ensure optimal pH, facilitate DNA binding, allow for easy observation of undissolved agarose gel.

  

• Ordering Information

  Cat No.	Size	Remark
  W70150-100	100 prep
  W70150-300	300 prep

• Features

  Contents	100 prep	300 prep
  GP Buffer	60 ml	180 ml
  Wash Buffer	16 ml	50 ml
  Elution Buffer	10 ml	30 ml
  Spin Columns	100 ea	300 ea
  Collection Tubes (2.0 ml)	100 ea	300 ea

• Technical Data

  

• Applications

  • PCR clean up
  • Gel Extraction

• Storage & Shipping

  Room temperature