DNA PurificationDNA/RNA PurificationWizPrep™ Gel/PCR Purification Mini Kit

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Açıklama

WizPrep™ Gel/PCR Purification Mini Kit was designed to recover or concentrate DNA fragments (100 bp→10 Kb) from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to dissolve agarose gel and denature enzymes.
DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. The unique dual-purpose application and high yield DNA/ minicolumn make this kit an exceptional value.
The method uses a chaotropic salt, guanidine thiocyanate to dissolve agarose gel and denature enzymes. DNA fragments in chaotropic salt solution bind to the glass fiber matrix of the spin column. Following washing off of contaminants, the purified DNA fragments are eluted by the addition of low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without phenol extraction or alcohol precipitation.

  • Overview
    • Optimized for fast and efficient DNA isolation based on silica membrane spin columns
    • High Efficiency : up to 90% recovery from agarose gels up to 95% recovery from PCR products or other enzymatic reactions
    • Convenient : gel extraction and PCR cleanup in one
    • Fast protocol : 10 minute
    • Broad Fragment Size Range: 100 bp ~ 20 kb
    • pH indicator is included in the GP Buffer to ensure optimal pH, facilitate DNA binding, allow for easy observation of undissolved agarose gel.

  • Ordering Information
    Cat No. Size Remark
    W70150-100 100 prep
    W70150-300 300 prep
  • Features
    Contents 100 prep 300 prep
    GP Buffer 60 ml 180 ml
    Wash Buffer 16 ml 50 ml
    Elution Buffer 10 ml 30 ml
    Spin Columns 100 ea 300 ea
    Collection Tubes (2.0 ml) 100 ea 300 ea
  • Technical Data
  • Applications
    • PCR clean up
    • Gel Extraction
  • Storage & Shipping
    Room temperature

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