Açıklama
• Deoxynucleotide triphosphates (dNTPs) are functionally tested in long PCR to be PCR qualified and meet or exceed criteria for high-quality sequencing with Thermo Sequenase DNA polymerase.
• High purity: 98% triphosphate, as confirmed by chromatographic analysis high levels of incorporation. It´s supplied as a buffer-free, ready-to-use solution and is available as individual products, sets, and premixes.
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Overview
- Advantages
- Greater than 99% purity confirmed by HPLC
- Free of human and E. coli DNA
Quality Control Analysis
- Purity : > 99.9% triphosphate.
- Base Purity : > 99.5% deoxynucleoside.
- Pyrophosphate : 0.003 pmol/pmol nucleotide.
- ATP : None detected
- Advantages
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Ordering Information
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Cat No. Description Size Remark W3120 dNTP mix (2.5 mM each) 1 ml W3121 dATP (100 mM) 1 ml W3122 dCTP (100 mM) 1 ml W3123 dGTP (100 mM) 1 ml W3124 dTTP (100 mM) 1 ml W3125 dUTP (100 mM) 1 ml W3126 dNTP set (100 mM each) 4 x 1 ml
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Applications
- Molecular biology applications, including amplification, dideoxy sequencing, labeling, mutagenesis, cDNA synthesis, RNA protection assay, expression profiling, PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension, DNA labeling, and more.
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Storage & Shipping
- Store at -20ºC in a frost-free freezer
Shipped on ice packs and/or dry ice
- Store at -20ºC in a frost-free freezer
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