Açıklama
WizScript™ cDNA Synthesis Kit (High Capacity) is a complete system for the efficient synthesis of high capacity cDNA from RNA templates. The recombinant Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation. The kit is also supplied with random primers.
The first strand of cDNA can be directly used as a template in PCR.
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Overview
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- Total Kit for cDNA synthesis
- Broad dynamic range
- Highly efficient synthesis of first-strand cDNA
- Thermostable RTase with RNase H minus
- Wide temperature range : 42 ~ 55°C
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- Ordering Information
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Cat No. Size Remark W2210 200 rxn without RNase Inhibitor W2211 200 rxn with RNase Inhibitor
- Features
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Contents W2210 W2211 WizScript™ RTase (200U/µl) 200 µl 200 µl 10X Reaction Buffer 1 ml 1 ml RNase Inhibitor (40U/µl) – 100 µl 20X dNTP mix (2.5mM) 200 µl 200 µl Random hexamer (50pM) 400 µl 400 µl RNase free water 1 ml 1 ml
- Technical Data
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Application
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- First Strand cDNA synthesis for RT-PCR
- Construction of cDNA libraries
- Generation of probes for hybridization
- Antisense RNA synthesis
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Storage & Shipping
- Store at -20ºC in a frost-free freezer
Shipped on ice packs and/or dry ice
- Store at -20ºC in a frost-free freezer
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