cDNA SynthesisPCRWizScript™ cDNA Synthesis Kit (High Capacity)

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Açıklama

WizScript™ cDNA Synthesis Kit (High Capacity) is a complete system for the efficient synthesis of high capacity cDNA from RNA templates. The recombinant Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation. The kit is also supplied with random primers.
The first strand of cDNA can be directly used as a template in PCR.

  • Overview
    • Total Kit for cDNA synthesis
    • Broad dynamic range
    • Highly efficient synthesis of first-strand cDNA
    • Thermostable RTase with RNase H minus
    • Wide temperature range : 42 ~ 55°C
  • Ordering Information
Cat No. Size Remark
W2210 200 rxn without RNase Inhibitor
W2211 200 rxn with RNase Inhibitor
  • Features
Contents W2210 W2211
WizScript™ RTase (200U/µl) 200 µl 200 µl
10X Reaction Buffer 1 ml 1 ml
RNase Inhibitor (40U/µl) 100 µl
20X dNTP mix (2.5mM) 200 µl 200 µl
Random hexamer (50pM) 400 µl 400 µl
RNase free water 1 ml 1 ml
  • Technical Data

Technical Data

  • Application
    • First Strand cDNA synthesis for RT-PCR
    • Construction of cDNA libraries
    • Generation of probes for hybridization
    • Antisense RNA synthesis
  • Storage & Shipping
    Store at -20ºC in a frost-free freezer
    Shipped on ice packs and/or dry ice

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